Sirius luminometer

The most versatile single tube luminometer, Sirius provides top of the line performance and flexibility for basic and applied research. The USB port connectivity,  comprehensive on-board software, high precision automatic reagent injectors and flexible PC software allow Sirius to meet the individual requirements of each  user.

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 Sirius applications include, but are not limited to:

Reporter Gene Assays 

Dual Reporter Assays

ATP Assays

Aequorin- based Ca2+Assays 

Toxicity Assays

Mycoplasma Detection Assays

Phagocyte Function Assays

Technical Data

Detector

Photomultiplier tube in photon counting mode,
spectral range 300-600 nm.

Measuring Chamber

Retractable drawer with interchangeable reflectors and
sample adaptors, running on roller bearings.

Sample Formats

Tubes up to 12 mm in diameter ,  microfuge tubes*, 35 mm culture dishes*, liquid scintillation vials up to 20 ml*.

Sensitivity

Better than 1000 molecules of luciferase in a reporter
gene assay. ATP detection limits set only by reagent quality.

Operating Software
(MicroProcessor)

Operating via three softkeys.
Measuring and delay time set in increments of 0.1s.
  Injector settings and flexible priming routine

  Single and Dual measurement mode

  Protocols: Raw Data, Single Assay, Cut-off, Dual Assay

  Background subtraction, average between replicates and standard error calculations. Ratio calculations for dual assays

Language choice: English or German.

Power Requirements

12 V,  2.5 mA supplied through main adaptor (included).

Interfaces

Serial (RS-232)  and  USB .

Dimensions

H: 23 cm (without injector), W: 32 cm, D: 20 cm

Weight

3.9 kg   Shipping weight (dimensional): 30 LB

Packing

18" x 18" x 18"

Injectors:                                                                                           

Design

Microprocessor controlled bellow-type injectors for
optimal control of injection profile and ease of use.

Number of Injectors

0, 1, 2 (upgrading possible)

Injection Volume

20 - 300 µL in increments of 10 µL

Precision
(SD in one series)

20 µL: 1%; 100 µL < 0.5%; 300 µL < 0.1%

Accuracy

+/- 3 µL

Tubing

Chemically inert PTFE tubing and connections (PTFE or
KEL-F), easy exchangeable liquid handling system and tips

* Can be used without injectors only. In injection mode 55 mm x 12 mm dia tube or 75 mm x 12 mm dia. tube must be used. 

PC software

Required Hardware

IBM Compatible PC, Pentium-like Processor; one
available serial or USB port

Operating System

Windows® XP, Vista

Additional Software

Microsoft® ExcelTM;  (optional)

Standard Configuration

Protocol Manager, Quick Measurement

Additionally Available

Single Assay, Dual Assay, Single Kinetics,
Multiple Kinetics, Cut-off Assay

Windows Features Provided

Full OLE support, fully resizable grids,
non-contiguous areas selectable, multiple
document interface, resizeable, zoomable
graphs, status bar, draggable toolbars, etc.

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